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Arxula - Yeast Bisphenol Screen

Detection of estrogenic effects


from 370 €
Test Specification
Duration of Assay
ca. 26 h
Number of Samples
max. 40 (BPAEQ)
Number of Samples (LID)
Calibration Range
0 - 100 µg/L (BPA)
Limit of Detection
6.4 µg/L (BPA)
Concerned about data analysis?







  •  Comes with our test kits
  •  Web tool with free access
  •  Simple and intuitive use
  •  Statistical assessment of results
  •  Comprehensive report with graphics
  •  Customized report available


Further documents


A-YBS data sheet


Technical requirements



Available test kit format



Other kits

Product Description
The A-YBS (Arxula-Yeast Bisphenol Screen) is an effect-based 96well assay for the detection of cumulative activity of Bisphenols in various types of water samples. The A-YBS uses the non-conventional and recombinant yeast Arxula adeninivorans as the bisphenol responsive biosensor. No sterile conditions are required for the whole test procedure.
Key Characteristics

The A-YBS determines the BPAEQ (bisphenol A Equivalents) of aqueous samples. This test is sensitive and specific for the detection of bisphenols especially bisphenol A and bisphenol Z.

Validation and Standardization

A factorial in-house validation was performed for method validation of the A-YBS.

Data Evaluation

Concerned about data analysis? With the purchase of our test kits you get free access to BioVAL for the statistical analysis and reporting of your test results.


Will this test fits to my purposes? We give sufficient support for choosing the right test for your application as well as before and during test establishment and for data evaluation.

Type of samples
Aqueous extracts
Ultrapure-, drinking- and mineral water
Surface water
Ground water and well water

Test Principle
Genetic Modifications

In the yeast genome the gene for directly modificated1 human estrogen receptor (hERα) and a reporter gene cassette have been integrated.


Estrogens and estrogenically active substances bind to the receptors and the resulting complexes trigger the transcription of the reporter gene.


The reference standard for calibration and quantification is 17β-Estradiol (E2). The dose-response curve corresponds to a non-linear model.


The detection is performed photometrical after reaction of the reporter enzyme with the chromogenic substrate p-nitrophenyl phosphate at 405 nm.

1 Johanna Rajasärkkä, Marko Virta; Characterization of a Bisphenol A Specific Yeast Bioreporter Utilizing the Bisphenol A-Targeted Receptor; Anal. Chem., 2013, 85 (21), pp 10067–10074