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Arxula - Yeast Dioxin Screen

Detection of Ah-Receptor mediated effects


320 €
Test Specification
Duration of Assay
ca. 26 h
Number of Samples
max. 40 (NEQ)
Number of Samples (LID)
Calibration Range
0-10,000 ng/L (β-NF)
Limit of Detection
59.0 ng/L (β-NF)
Concerned about data analysis?







  •  comes with our test kits
  •  web tool with free access
  •  simple and intuitive use
  •  statistical assessment of results
  •  comprehensive report with graphics
  •  customized report available


Further documents


A-YDS data sheet


Technical Requirements



Available test kit format



Other kits

Product Description
The A-YDS (Arxula-Yeast Dioxin Screen) is an effect-based 96well assay for the detection of aryl hydrocarbon receptor mediated activity in surface water and aqueous extracts. The A-YDS uses the non-conventional and recombinant yeast Arxula adeninivorans as the responsive biosensor for aryl hydrocarbon receptor mediated activity. No sterile conditions are required for the whole test procedure.
Key Characteristics

The A-YDS determines the NEQ (β-Naphthoflavone Equivalents) of water samples.

Validation and Standardization

An in-house validation was performed for method validation.

Data Evaluation

Concerned about data analysis? With the purchase of our test kits you get free access to BioVAL for the statistical analysis and reporting of your test results.


Will this test fits to my purposes? We give sufficient support for choosing the right test for your application as well as before and during test establishment and for data evaluation.

Type of samples
Determination aryl hydrocarbon receptor mediated effects of drug candidates
Ultrapure, drinking and mineral water
Surface water
Ground water and well water
Aqueous extracts

Test Principle
Genetic Modifications

In the yeast genome the genes for the human aryl-hydrocarbon receptor (hAhR) and the human aryl-hydrocarbon receptor nuclear translocator (hARNT) and a reporter gene cassette have been integrated.


In the presence of hAhR ligands, heterodimeric complexes are formed which act as transcription factors for the transcription of the reporter gene.


Calibration The reference standard for calibration and quantification is β-Naphthoflavone (β-NF). The dose-response curve corresponds to a non-linear model.


The detection is performed photometrical after reaction of the reporter enzyme with the chromogenic substrate p-nitrophenyl phosphate at 405 nm.