Arxula - Yeast Mineralcorticoid Screen
Detection of mineralcorticoid effects
The A-YMS determines the CLEQ (Cortisol Equivalents) of aqueous samples.
A factorial in-house validation was performed for method validation of the A-YMS..
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In the yeast genome the gene for the human mineralcorticoid receptor (NR3C2) and a reporter gene cassette have been integrated.
Mineralcorticoids bind to the receptors and the resulting complexes trigger the transcription of the reporter gene.
The reference standard for calibration and quantification is cortisol (CL). The dose-response curve corresponds to a non-linear model.
The detection is performed after reaction of the reporter enzyme phytase with the chromogenic substrate p-nitrophenyl phosphate at 405 nm.